An increasing focus on the efficacy of natural products to combat infectious agents is driven by a need for alternative approaches and consumer demand for natural products to avoid side effects. The coronavirus pandemic has heightened public awareness of transmission of disease and methods to reduce transmission either through social distancing, mask waring, hand hygiene and regular cleaning of high touch surfaces are now commonplace. Naturally, sourced products with demonstrated anti-viral efficacy are popular due to minimum association with side effects. This project will provide a comprehensive analysis of the anti-viral properties of Tea Tree oil using the appropriate methodology that is recognized by the Australian Therapeutic Drugs Administration (TGA). The project will the test the efficacy of tea tree oil against a range of virus types with an emphasis on viruses that are transmitted by the respiratory route or by encountering contaminated surfaces. This project will place an emphasis on measuring and validating the anti-viral activity of tea tree oil against coronaviruses and will also perform experiments to replicate the virucidal effect of tea tree oil as a disinfectant for contaminated surfaces. Furthermore, the project will test for anti-viral activity against animal viruses also associated with respiratory disease such as bovine herpes virus (Bovine respiratory disease) and canine coronavirus (Kennel cough), providing further information for potential use of tea tree oil in veterinary applications. The main project outcome will be a high impact publication serving as a reference study for tea tree oil anti-viral activity.