This project provides a rigorous evidence base for the value of Australian Leptospermum honey as a therapeutic agent that is effective against bacteria. It undertakes a comprehensive and systematic survey of Leptospermum honey from throughout the major beekeeping areas of Australia. The survey includes a chemical and antimicrobial analysis of these honeys to identify those that have the highest antibacterial activity as correlated with MGO/DHA levels. Honeys are assessed for broadspectrum antibacterial activity and tested for synergy when honey is combined with commonly used antifungals and antibacterials.